Reconstructing diploid 3D chromatin structures from single cell Hi-C data with a polymer-based approach

Reconstructing diploid 3D chromatin structures from single cell Hi-C data with a polymer-based approach

Blog Article

Detailed understanding of Flywheel Assembly the 3D structure of chromatin is a key ingredient to investigate a variety of processes inside the cell.Since direct methods to experimentally ascertain these structures lack the desired spatial fidelity, computational inference methods based on single cell Hi-C data have gained significant interest.Here, we develop a progressive simulation protocol to iteratively improve the resolution of predicted interphase structures by maximum-likelihood association of ambiguous 24" Warming Drawer Hi-C contacts using lower-resolution predictions.

Compared to state-of-the-art methods, our procedure is not limited to haploid cell data and allows us to reach a resolution of up to 5,000 base pairs per bead.High resolution chromatin models grant access to a multitude of structural phenomena.Exemplarily, we verify the formation of chromosome territories and holes near aggregated chromocenters as well as the inversion of the CpG content for rod photoreceptor cells.